Induction of Antinuclear Antibodies by De Novo Autoimmune Hepatitis Regulates Alloimmune Responses in Rat Liver Transplantation
Figure 3
The induction of Ab against nuclear histone H1 (a) and HMGB1 (b) after OLT by the transient induction of de novo autoimmune hepatitis. Anti-histone H1 and HMGB1 Ab titers (optical density at 405 nm) were measured by ELISA as described in the Materials and Methods section. The results are expressed as the mean of three independent experiments (ratio to day 0) ± SD. The dotted line indicates cutoff values () of 2SD above the mean of baseline () (a: 1.35; b: 1.25). (c) No elevation or transient induction of anti-histone H1 Ab titer was observed in the serum of DA-LEW OLT without Con A injection or nontransplanted LEW rats with Con A injection. The results are expressed as the mean of three independent experiments (ratio to naïve LEW) ± SD. **Significantly different compared with the naïve LEW rats () (, Student’s t-test).
