The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Figure 6
The desensitized PAFR is not reactivated by CytB. Neutrophil was desensitized with fMLF (100 nM; FPR1 agonist, solid line) or PAF (100 nM; PAFR agonist, dashed line) at 15°C for 10 min. The cells were then transferred to 37°C, and the incubation was continued for another 10 min. The cells were then challenged with CytB (5 μg/mL, added at the time point indicated by arrow), and reactivation of the receptor was determined through the release of superoxide. One representative experiment out of three is shown. Abscissa: time of study (min); ordinate, superoxide production expessed as counts per minute 106 (Mcpm).
