Research Article

The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics

Figure 7

PAF induces a transient rise in intracellular Ca2+ in neutrophils. Fura-2 loaded neutrophils (2 × 106/mL) were triggered with different concentrations of PAF (a) or fMLF (b), and the changes in fluorescence were followed using dual excitation at 340 nm and 380 nm, respectively, and an emission wavelength of 510 nm. A representative experiment out of at least three is shown, and an increase in Ca2+ is visualized as an increase in fluorescence when excited at 340 nm. To make a direct comparison easy, curves obtained with the different agonist concentrations are shown on top of each other. Abscissa, time of study (sec). The time point for addition of an agonist is marked by arrow. Inset: mean values of the transient rise in [Ca2+]i (arbitrary units ± SEM ( )).
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(a)
456407.fig.007b
(b)