Research Article

The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration

Figure 2

Anti-CD3 and IL-7 stimulation upregulates Netrin-1 and Unc5b expression on thymocytes. ((a) and (b)) RT-PCR showing an increase in Netrin-1 gene expression on thymocytes following anti-CD3 stimulation. Histograms represent the Netrin-1/actin ratio. (c) Real-time PCR showing Netrin-1 expression on thymocyte subsets, DN, DP, CD4SP, and CD8SP before and after anti-CD3 stimulation. (d) Enzyme-linked immunosorbent assay of Netrin-1 in conditioned medium treated with anti-CD3 (5  g/mL). (e) Real-time PCR showing Netrin-1 and Unc5b expression by IL-7-treated (12 h) or untreated total thymocytes. (f) Real-time PCR showing an increase in Unc5b gene expression on thymocytes following anti-CD3 stimulation. Data shown in each image are representative of three independent experiments. NS: not significant; ; ; .