Research Article

Effects of Muscarinic Acetylcholine 3 Receptor208-227 Peptide Immunization on Autoimmune Response in Nonobese Diabetic Mice

Figure 3

The secretions of IL-17, IL-4, IL-6, and TGF- were decreased in the cell supernatants following incubation with M3R208–227 peptide. Spleens were isolated from the NOD/LtJ mice immunized with the M3R208–227 peptide and the spleen cells were pooled. The homogenates were adjusted to 1 × 106 cells/mL and incubated with M3R208–227 (black bar), CP (charcoal gray bar), or PBS (white bar). Untreated spleen cells served as an additional control (light gray bar). Cell supernatants were collected after 48 hours of culture, and the cytokine concentrations were measured using ELISA kits. (a) The results indicated that the secretion of IL-17 was significantly reduced in the supernatants of cells that had been cocultured with the M3R208–227 peptide in comparison to those cocultured with CP and PBS or left untreated ( ), although no significant decrease in IFN-γ was observed ( ). (b) These results showed that the secretion of IL-4 was also significantly decreased in the supernatants of cells cocultured with M3R208–227 as compared to those cocultured with CP and PBS or left untreated ( ). No significant decrease in IL-10 secretion was observed ( ). (c) The results showed that the secretion of IL-6 and TGF- was significantly decreased in the supernatants of cells cocultured with M3R208–227 as compared to those cocultured with CP and PBS or left untreated ( ; ).
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(a)
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(c)