Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Protein</td><td align="center">Restriction sites</td><td align="left">Primer sequences (5<sup>'</sup>–3<sup>'</sup>)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left" rowspan="2">SLA-His-coli</td><td align="center">BsmBI</td><td align="left">F: ATTACGTCTCACATGAACCGCGAGAGCTTCGCGGCG</td></tr><tr><td align="center">BsmBI</td><td align="left">R: ATTACGTCTCTTCGAGTGAAGAAGCATCCTGGTATGTGTC</td></tr><tr><td align="left" colspan="3"><hr/></td></tr><tr><td align="left" rowspan="2">SLA-HEK</td><td align="center">BsmBI</td><td align="left">F: ATTACGTCTCACATGAACCGCGAGAGCTTCGCGGCG</td></tr><tr><td align="center">BsmBI</td><td align="left">R: ATTACGTCTCTTCGAGTCATGAAGAAGCATCCTGGTATGTG</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Primer sequences for PCR amplification of cDNA fragments of SLA. Primers were synthesized by MWG, Germany. F: forward primer; R: reverse primer.

Journal of Immunology Research

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Table 1

Table 1: Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis 

Table 1 | Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis 