Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant
Analyses of splenocyte T-cell subsets. (a) Percentage of the CD3+, CD4+, and CD8+ T-cell subsets in splenocytes of experimental mice measured by flow cytometry. The splenocytes of the hyperglycemic PBS (, median and average blood glucose 33 mmol/L, median age 21 wks, average 21.25 wks of age), euglycemic CFA (1 : 0) ( median 8.05 mmol/L, average 7.88 mmol/L, median/average 119 wks), rVV-CTB::GAD + CFA (1 : 0) (, median 7.7 mmol/L, average 7.77 mmol/L, median/average 116 wks), and rVV-CTB::GAD + CFA (1 : 10) ( median 6.8 mmol/L average 6.43 mmol/L, median 119 wks, average 117 wks) treated mice were analyzed after stimulation with GAD65. Differences between percentage of the CD4+ T-cell subsets of the gated cell population of the mice inoculated with rVV-CTB::GAD + CFA (1 : 10) compared to PBS and CFA (1 : 0) are statistically significant ( and 0.018, resp.; one-way Anova). (b) Example of the relationship between the percentage of the CD4+ and CD8+ T-cell subsets of the gated cell population of the mice inoculated with CFA (1 : 0) and rVV-CTB::GAD + CFA (1 : 10). Cells were stained with antibodies for CD4 and CD8, as described in Section 2, and analyzed by flow cytometry. (The percentages of cells in quadrant, are shown relative to gated cells. Representative data from one mouse is displayed.)
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