Expression and Function of the Homeostatic Molecule Del-1 in Endothelial Cells and the Periodontal Tissue
Figure 5
Regulation of Del-1 versus ICAM-1 and VCAM-1 by inflammatory stimuli. HUVEC were cultured for the indicated time intervals with 10 ng/mL TNF (a), 5 μg/mL serum amyloid A (SAA; (b)), P. gingivalis (Pg; MOI = 10 : 1) (c), Pam3CSK4 or LPS (both at 0.5 μg/mL; (d)), 10 ng/mL IFNγ (e), or IL-17A or IL-17F (both at 10 ng/mL; (f)) and assayed for Del-1, ICAM-1, and VCAM-1 mRNA expression. Results were normalized to those of GAPDH mRNA and expressed as fold change in transcript levels relative to those of medium-treated cells at 2 hours (HRS), the average value of which was taken as 1. The medium-treated groups in (b) and (c) are the same (SAA and Pg were tested together but were separated in the graphs for enhanced clarity). Data are means ± SD of duplicate determinations from one of three independent sets of experiments that yielded similar results.
