Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays

Table 1

Variants of cultured ELISPOT protocols.

Reference	Length of culture	Size of culture plate	Cells per well in culture plate	Concentration and addition of IL-2	Washing and resting cells before ELISPOT
Godkin et al. 2002 [37]	12 days	96-well plate	2×10^5	10 U/mL on days 3, 6, and 9	Washing cells only
Pinder et al. 2004 [38]	14 days	Not described	1×10^6	10 U/mL on days 5 and 10	Washing cells only
Reece et al. 2004 [44]	14 days	48-well plate	1×10^6	10 U/mL on days 5 and 10	Washing cells only
Keating et al. 2005 [32]	10 days	24-well plate	1×10^6	10 U/mL on days 3 and 7	Washing and resting cells overnight
Goonetilleke et al. 2006 [35]	11 to 13 days	Not described	2×10^6	1800 U/mL on days 3 and 7	Washing and resting cells overnight
Calarota et al. 2008 [33]	12 days	24-well plate	5×10^5	10 U/mL on days 3 and 7	Washing cells only
Todryk et al. 2009 [40]	10 days	24-well plate	1×10^6	100 U/mL on days 3 and 7	Washing and resting cells overnight