Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study
Figure 1
(a) Levels of IgE, IgG1, and IgG4 antibodies to Dermatophagoides pteronyssinus (Dpt) allergen extract in serum samples from atopic and nonatopic patients. Data are expressed in ELISA index (EI), and mean is indicated by horizontal bars. The dashed line indicates the cutoff of the reaction (). Percentages of positive samples are also indicated. Statistically significant differences were determined by the Mann-Whitney test (; ). (b) Correlation between levels of Dpt-specific IgE versus IgG1 and IgE versus IgG4 anti-Dpt in serum samples from atopic patients. Percentages of double positive, double negative, or single positive for each antibody class are indicated in the correspondent corners. Spearman’s correlation coefficient and statistical significance are also indicated. (c)Comparison between levels of IgG1 and IgG4 anti-Dpt in serum samples from atopic and non-atopic patients. Five serum samples (red ellipses) of each patient group were selected to constitute the specific (atopic) and nonspecific (nonatopic) total IgG pools. The dashed lines indicate the cutoff of the reaction ().
