Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study

Figure 2

(a) Slot-blots showing reactivity for IgE, IgA, IgG, and IgM in the serum, supernatant (S 40%), and precipitated (P 40%) fractions obtained from precipitation of the serum with 40% ammonium sulfate solution, and bovine serum albumin (BSA) as irrelevant protein. (b) Slot-blot data analysis by measuring the intensity of the bands in gray scale and expressed in arbitrary units (AU). Statistically significant differences were determined by Student’s 𝑡 test (^∗∗𝑃<0.01; ^∗∗𝑃<0.001).

(a)

(b)