Trichostatin A Promotes the Generation and Suppressive Functions of Regulatory T Cells
Figure 1
The foxp3 promoter is hyperacetylated on iTreg cells. (a) Flow cytometry of the sorted naive cells and in vitro differentiated Treg and Th17 cells. Numbers indicate the percentage of naive (CD4+CD25ā), Treg (CD4+Foxp3+), and Th17 (CD4+IL-17+) positive cells. (b) chromatin immunoprecipitation assays on foxp3 promoter. ChIP assays were performed on sorted splenic naive CD4+ T cells and in vitro induced Tregs and Th17 cells. DNA fragments bound to acetylated histones were immunoprecipitated using antibodies directed against acetylated histone H3 (H3ac) or a rabbit isotype-matched immunoglobulin G (IgG), as control. Precipitated DNA was quantified by real-time PCR with primers specific for the foxp3 gene promoter, and the PCR products were set in relation to input DNA. Standard deviation was obtained from three (naive and iTregs) and two (iTh17) independent experiments.
