Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis
Figure 2
Frequency of T cell populations expressing costimulatory molecules. Frequency of T (a), T (b), and T cells (c) in individuals infected with S. mansoni with different degrees of periportal fibrosis. Ex vivo PBMC were stained for surface markers. The selection of T lymphocyte subsets was initially performed based on their forward (FSC) and side (SSC) light scatter properties, followed by immunophenotyping using a double-labeling platform with anti-CD4-FITC, anti-CD28-PE, anti-CD69-PerCP, and anti-CD152-PeCy5 mAbs and flow cytometry. Results are expressed in median, minimum, maximum, and interquartile values of frequency (*, **; Kruskal-Wallis test).