Research Article

Hepatic Expression of Metallothionein I/II, Glycoprotein 96, IL-6, and TGF-β in Rat Strains with Different Susceptibilities to Experimental Autoimmune Encephalomyelitis

Figure 4

Expression of TGF-β in the livers of EAE-prone DA and EAE-resistant AO rats. (a) Representative western blots indicate the TGF-β protein (~44 kD; range 35–55 kD), detected by mouse anti-rat antibody to IL-6 in the liver samples obtained from intact rats and rats treated by bovine brain homogenate (BBH) and complete Freund’s adjuvant (CFA) or by CFA at the time of first attack in EAE-prone rats (day 12). As a loading control the blots of β-actin protein (37–50 kD) are shown. (b) Relative protein expression is shown as AO/DA ratio (intact liver) and as the percent of control (liver in EAE). All band densities were normalized corresponding to -actin. Analyses were made in tissue samples, prepared as a pool from 3 rats in three independent experiments. Data represent mean ± SE.