Journal of Immunology Research / 2013 / Article / Fig 8

Research Article

Altered Sympathetic-to-Immune Cell Signaling via β2-Adrenergic Receptors in Adjuvant Arthritis

Figure 8

Expression of PKA-phosphorylated-β 2-AR (pβ 2-ARPKA)/β 2-ART in DLN cells from rats challenged with CFA, SMB, or MO compared with Saline-treated controls. Levels of pβ 2-ARPKA/β 2-ART were not significantly different in Saline- and MO-treated rats on D21 (a) or D28 (b). In contrast, pβ 2-ARPKA/β 2-ART in DLN cells from CFA- and SMB-challenged rats were significantly increased on D21, but significantly decreased on D28 compared with Saline- or MO-treated rats. DLN cells were harvested, lysed, and total protein collected and resolved by SDS-PAGE. Cellular extracts were probed with an antibody against the phosphorylated Ser345, Ser346 of the β 2-AR to determine pβ 2-ARPKA expression and quantified by densitometry. A western blot is shown (c) that is representative of the blots seen within each treatment. The data were normalized to β-actin, then pβ 2-ARPKA was normalized to β 2-ART levels. Each bar represents the mean pβ 2-ARPKA/β 2-ART  ± SEM with an of 8 rats per treatment group. Data were analyzed using one-way ANOVA with Bonferroni posthoc testing (* ; ** ; *** ).

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