LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice
Figure 5
The mRNA levels of leukocyte activation markers in nucleosome- and nucleosome/heparin-stimulated splenocytes from a nephritic mouse. The mRNA levels of CD80 (a), CD86 (b), CD69 (c), CTLA-4 (d), CD25 (e), and CD19 (f) are all upregulated over time in response to stimuli with nucleosomes and nucleosomes/heparin. Expression levels for each gene, measured by qPCR, were normalized against the expression level of TBP. Data are given as Log 2 of mean ± SEM of fold change values normalized against medium stimulated cells and are representative for at least three independent analyses. Fold change is shown as mean of triplicates ± SD. *Statistically significant () change in mRNA levels compared to medium stimulated cells at the same time point.
