Journal of Immunology Research / 2013 / Article / Fig 2

Research Article

An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model

Figure 2

One-step growth curve analysis of recombinant virus v545. Wild-type (ATCC 22122), recombinant (v545) and rescuant (v545R) viruses were used to infect confluent cell culture monolayers at an m.o.i. of 0.5 pfu/cell. After absorption for 1 h at 37°C, the cells were washed with media and a zero time point was harvested. The remainder of the sample was incubated at 37°C with additional time points taken at indicated times. The viral titer of each time point was determined by plaque titration assay on GPL cells.

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