Journal of Immunology Research / 2013 / Article / Fig 5

Research Article

An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model

Figure 5

Immunogenicity of v545 attenuated vaccine. (a) ELISA profile following vaccination. Animals were immunized twice, with an interval of 3 weeks between doses, with  pfu of v545 vaccine, by subcutaneous route of inoculation. Control animals received an identical volume of phosphate-buffered saline. Bleeds were performed at regularly intervals, as described in the text, on all guinea pigs following vaccination for serum ELISA analysis. Selected sera were also used in western blot assay as indicated in panel (b). Data shown represents the mean ± SD reciprocal ELISA titer for the vaccine group at each time point. (b) Representative profile of response from vaccinated dam using viral particles in western assay. Marker lane is indicated (M). Lanes 1–4, preimmune sera and sera from weeks 2, 5, and 11 following vaccine. Lane 5, western profile of high-titer anti-GPCMV antisera. Lane 6, western blot with anti-GPCMV gB polyclonal antibody. Immunized animals demonstrate anti-gB antibodies (arrowhead) in addition to antibody to other GPCMV polypeptides. Initial response targets virion proteins of ~35 and 45 kDa noted at 2 weeks after vaccination, followed by responses to gB and a ~70 kDa protein following second immunization at 5-week time point. Last serological responses to appear target proteins of ~22 kDa, noted at 11-week time point.

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