Figure 1: Peptide stimulation step. (a) 107 PBMC from HLA-A2 healthy donors were stimulated in 96-well plated (containing 2 × 105 cells/well) for 14 days with Melan-A or MELOE-1 peptides. (b) Percentages of Melan-A- or (c) MELOE-1-specific T cells detected by double labelling with tetramer and anti-CD8 antibody, in microcultures stimulated with 0.1 to 10 μM of Melan- or MELOE-136-44. Each symbol corresponds to a microculture containing more than 0.5% of specific T cells.