Research Article

A Full GMP Process to Select and Amplify Epitope-Specific T Lymphocytes for Adoptive Immunotherapy of Metastatic Melanoma

Figure 2

Influence of IL-2 concentration on the efficiency of peptide stimulation. 107 PBMC from HLA-A2 healthy donors were stimulated in 96-well plated (2 × 105 cells/well) with either Melan- peptide (1 μM) or the MELOE-136-44 peptide (10 μM). (a) Percentages of Melan-A- or (b) MELOE-1-specific T cells detected by double labelling with tetramer and anti-CD8 antibody, in microcultures stimulated in the presence of various IL-2 concentrations. Each symbol corresponds to a microculture containing more than 0.5% of specific T cells.
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(a)
932318.fig.002b
(b)