Figure 2: Influence of IL-2 concentration on the efficiency of peptide stimulation. 107 PBMC from HLA-A2 healthy donors were stimulated in 96-well plated (2 × 105 cells/well) with either Melan- peptide (1 μM) or the MELOE-136-44 peptide (10 μM). (a) Percentages of Melan-A- or (b) MELOE-1-specific T cells detected by double labelling with tetramer and anti-CD8 antibody, in microcultures stimulated in the presence of various IL-2 concentrations. Each symbol corresponds to a microculture containing more than 0.5% of specific T cells.