Figure 7: Design of the whole GMP process. At day 0, 100 mL of blood is collected from HLA-A2 melanoma patients. Total PBMC are stimulated with the two antigenic peptides during 14 days. At day 14, antigen-specific T cells are sorted using Clinimers, and 105 rosetted T cells are seeded for amplification on feeder cells. At day 28, purity of amplified cells is assessed by flow cytometry and between 108 and 5 × 108 T lymphocytes specific for each peptide will be infused to the autologous melanoma patient.