Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain
Figure 1
PC61 treatment specifically eliminates brain-infiltrating CD4+CD25hiFoxp3+ Treg. (a) Two weeks after tumor challenge, animals that were either prophylactically treated with PC61 anti-CD25 mAb (black bars, ) or with control rat IgG (grey bars, ) were sacrificed, and the brain-infiltrating CD4+ and CD8+ lymphocytes were analyzed by flow cytometry for expression of CD25 and Foxp3. Results are presented as absolute numbers. (b) Kaplan-Meier survival graph with PC61-treated mice (solid line, ) and rat IgG control mice (dotted line, ). Mice were tumor challenged on day 0. In one experiment, mice were challenged with Fluc-transduced GL261 cells. (c) Tumor burden was assessed through bioluminescence imaging on days 4 and 12 after tumor challenge of both PC61-treated mice (solid lines, ) and control IgG-treated mice (dashed lines, ). Data are presented as LOG (peak flux).
