Inflammatory Chemokine Expression via Toll-Like Receptor 3 Signaling in Normal Human Mesangial Cells
Figure 1
Knockdown of RIG-I reduces the poly IC-induced expression of CCL5 in human MCs. The cells were transfected with siRNA against RIG-I or control siRNA and then stimulated with 20 μg/mL of poly IC (Imaizumi et al. NDT 2010). (a) After 24 h of poly IC treatment, the cells were lysed, and western blotting for CCL5 was performed. (b) The cells were incubated for 16 h with poly IC, RNA was extracted, and RT-PCR was performed for RIG-I, CCL5, and CCL2. (c) The culture medium was collected after 24 h, and the concentration of CCL5 was determined by ELISA (, ).
