Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy

Table 2

NOVA View agreement with manual IIF: 1 : 80 screening dilution.

All patients (𝑛=369)	Manual IIF			Percent agreement (95% confidence)
	Positive	Negative	Total
NOVA View
Positive	92	7	99	Positive agreement = 92.9% (86.0–97.1%)
Negative	7	263	270	Negative agreement = 97.4% (94.7–99.0%)
Total	99	270	369	Total agreement = 96.2% (93.7–97.9%)
				𝜅 = 0.90 (95% CI 0.85–0.95)

𝑁: number of patients tested at a 1 : 80 dilution for HEp-2 ANA antibodies.
Manual results were read on a Nikon Eclipse 400 fluorescent microscope with an LED light source.
NOVA View confirmed results = images reviewed and confirmed by the operator.