Regulatory Lymphocytes Are Key Factors in MHC-Independent Resistance to EAE
Figure 4
Intracellular production of interferon-gamma (IFN-gamma) by spleen CD4+ T cells. We analyzed these cells in series of seven mice per condition. (a) Splenocytes from C57BL/6 and CD1 mice were cultured in presence of phorbol-12-myristate-13-acetate (PMA) and ionomycin during four hours, and the percentage of CD4+ T cells showing intracellular production of IFN-gamma was analyzed by flow cytometry. We studied nonimmunized mice (NI) and mice immunized with MOG35–55 (MOG) and with PLP139–151 (PLP). C57BL/6 mice that developed EAE after immunization with MOG35–55 experienced a significant increase in the percentage of CD4+ T cells producing IFN-gamma. (b) Representative dot plots of IFN-gamma intracellular staining on CD4+ T splenocytes from CD1 and C57BL/6 mice upon immunization with MOG35–55.
