Research Article

Osteodifferentiated Mesenchymal Stem Cells from Bone Marrow and Adipose Tissue Express HLA-G and Display Immunomodulatory Properties in HLA-Mismatched Settings: Implications in Bone Repair Therapy

Figure 4

AT-derived MSCs committed to osteodifferentiation process express HLA-G and are hypoimmunogenic. (a) Expression of HLA-DR molecules was evaluated by flow cytometry analysis on AT-derived MSCs committed to 14-day osteodifferentiation process and pretreated with IFNγ and TNFα (Os-MSC-AT IFNγ/TNFα) or not (Os-MSC-AT). (b) Expression of ALP and HLA-G5 was evaluated by intracellular flow cytometry analysis on AT-derived MSCs committed to 14-day osteodifferentiation process and pretreated with IFNγ and TNFα (Os-MSC-AT IFNγ/TNFα) or not (Os-MSC-AT). IgG1 was used as isotype control Ab. (c) PBMC from healthy individual (#00) were used as responder cells towards AT-derived MSCs committed to osteodifferentiation process and pretreated with IFNγ and TNFα (Os-MSC-AT IFNγ/TNFα) or not (Os-MSC-AT) as stimulating cells at various responder : stimulator ratios. Irradiated were used as positive control of T cell alloproliferation. Results are given as mean cpm ± s.e.m.; one representative experiment is shown.
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