Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy

Figure 2

mRNA and protein expression of TGF-β1 and TRB3 upregulated in DN mice. (a) Protein TRB3 level in nucleus of intrinsic glomerular cells and tubular epithelial cells. Total TGF-β1 and TRB3 mRNA (b) and protein (c) levels by RT-PCR and western blot analysis, respectively. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus age-matched control mice. Scale bars, 50 μm. 𝑛=5 mice per group.

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