Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy

Figure 5

Effect of TRB3 on phosphorylated ERK1/2 (pERK1/2) expression in cells. (a) Western blot analysis of pERK1/2 level in cells incubated with HM or HG for 6, 12, 24, and 48 h. (b) Cells were transfected with TRB3 siRNA or corresponding negative control. Western blot analysis of pERK1/2 level with HG stimulation for 48 h. Data are mean ± SEM. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus NG, ^ΔΔ𝑃<0.01 versus HG, and ^#𝑃<0.05, ^##𝑃<0.01 versus NG +TRB3 siRNA.

(a)

(b)