Research Article
Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy
Table 1
Primers used for RT-PCR.
| | Name | GenBank accession | Primers | Size
(bp) |
| | TRB3 | NM 175093.2 | Forward: 5′-GCTCTGAGGCTCCAGGACAA-3′ | 91 | | Reverse: 5′-TGTCATCAAACTCCAACGGTTTC-3′ |
| | TGF- | NM 011577.1 | Forward: 5′-GTGTGGAGCAACATGTGGAACTCTA-3′ | 174 | | Reverse: 5′-CGCTGAATCGAAAGCCCTGTA-3′ |
| | Collagen type I | NM 007742.3 | Forward: 5′-CAACAGTCGCTTCACCTACAGC-3′ | 201 | | Reverse: 5′-GTGGAGGGAGTTTACACGAAGC-5′ |
| Collagen
Type IV | NM 009931.2 | Forward: 5′-TATGTCCAAGGCAACGAGC-5′ | 228 | | Reverse: 5′-AACCGCACACCTGCTAATG-3′ |
| | -Actin | NM 007393.3 | Forward: 5′-GTG ACG TTG ACA TCC GTA AAG A-3′ | 245 | | Reverse: 5′-GCC GGA CTC ATC GTA CTC C-3′ |
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