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Journal of Immunology Research
Volume 2014, Article ID 241246, 6 pages
Research Article

The Feature of Distribution and Clonality of TCR / Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma

1Department of Oncology, First Affiliated Hospital, Jinan University, Guangzhou 510632, China
2Centre of Oncology and Hematology, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510230, China
3Department of Rheumatism and Immunology, First Affiliated Hospital, Jinan University, Guangzhou 510632, China
4Institute of Hematology, Jinan University, Guangzhou 510632, China
5Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China

Received 30 March 2014; Accepted 3 May 2014; Published 21 May 2014

Academic Editor: Qintai Yang

Copyright © 2014 Liang Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Restricted T-cell receptor (TCR) V/V repertoire expression and clonal expansion of T cells especially for putative tumor-associated antigens were observed in patients with hematological malignancies. To further characterize the T-cell immune status in B-cell non-Hodgkin lymphoma (B-NHL), we investigated the distribution and clonality of TCR V/V repertoire in peripheral blood (PB), bone marrow (BM), and lymph node (LN) from patients with B-NHL. Four newly diagnosed B-NHL cases, including three with diffuse large B-cell lymphoma (DLBCL) and one with small lymphocytic lymphoma (SLL), were enrolled. The restrictive expression of TCR V/V subfamilies with different distribution patterns could be detected in PB, BM, or LN from all of four patients, and partial subfamily T cells showed clonal proliferation. At least one clonally expanded V subfamily member was found in PB from each patient. However, the expression pattern and clonality of TCR V/V changed in different immune organs and showed individual feature in different patients. The clonally expanded V5, V6, and V8 were detected only in PB but neither in BM nor LN while clonally expanded V2 and V3 could be detected in both PB and BM/LN. In conclusion, the results provide a preliminary profile of distribution and clonality of TCR subfamilies T cells in PB, BM, and LN from B-NHL; similar clonally expanded V subfamily T cells in PB and BM may be related to the same B-cell lymphoma-associated antigens, while the different reactive clonally expanded V/V T cells may be due to local immune response.