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Journal of Immunology Research
Volume 2014, Article ID 318098, 9 pages
Research Article

IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway

1Department of Gynecology and Obstetrics, First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China
2Kingmed College of Laboratory Medicine, Guangzhou Medical University, Guangzhou 510182, China
3Department of Gastrointestinal-Pancreatic Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 51800, China

Received 10 March 2014; Revised 5 May 2014; Accepted 5 May 2014; Published 26 May 2014

Academic Editor: Yi Zhang

Copyright © 2014 Xinyuan Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

After fixation and co-culture with fluorescence-labeled antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105 and CD166 (BD Pharmingen), respectively, the immunophenotypes of MSCs and MSCs IFN-γ were analyzed by flow cytometry..

The adipogenic and osteogenic differentiation of MSCs and MSCs IFN-γ were performed as described in Materials and Methods. At the end of induction, the differentiation capability of MSCs and MSCs IFN-γ were identified by oil red O and alizarin red S staining, respectively.

The same quantity (5x104/well) of MSCs and MSCs IFN-γ were plated and cultured for 48 h. at last, the cell number were measured by CCK-8 kit.

  1. Supplementary Material