Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Figure 10
Histochemical and immunohistochemical analysis of spleens from unchallenged and challenged mice from adjuvant only C57BL/6 wild-type, F1-V vaccinated C57BL/6 wild-type, and F1-V vaccinated C57BL/6 Myd88-deficient mice. Mice were obtained from the corresponding group of mice as described in Figures 7(b) and 9. Spleens from additional mice from each group were used as controls (Unchallenged-H&E, panels (a), (d), and (g)). Regions in the spleen were labeled: white pulp, WP; red pulp, RP; and marginal zone, MZ. Spleen sections from challenged mice are shown stained (Challenged-H&E, panels (b), (e), and (h)) or probed with an anti-F1 monoclonal antibody (Challenged-Anti-F1, panels (c), (f), and (i)). The spleen section shown from Group 2, wild-type+F1-V was from a mouse that survived challenge (panels (e) and (f)). Arrows in panel (c) and (i) point to F1 positive regions in the marginal zone. Arrows in panel (f ) point to isolated F1 positive spots.
