Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Figure 5
Cytokine expression by stimulated splenocytes from F1-V vaccinated wild-type C57BL/6 or MyD88-deficient mice appears to require MyD88. Splenocytes were stimulated with F1-V (5 μg) or medium alone for approximately 45 h before collecting the supernatant and determining the amount of cytokine present: (a), IFN-; (b), IL-4; (c), IL-10. Cells from three different groups of mice were used (see Figure 4): (1) wild-type (Wt) C57BL/6 mice that received only adjuvant, (2) Wt C57BL/6 mice that received F1-V (2 μg), and (3) Myd88-deficient C57BL/6 mice that received F1-V (2 μg). The results are reported as mean with standard error of the mean. Statistical significance shown above the respective bar was reported for differences between the amount of cytokine expressed between the wild-type C58BL/6 and Myd88-deficient splenocytes stimulated with F1-V ( and ) in panels (a) and (c), respectively.
