Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway

Figure 7

NF-κB p65 expression in human endothelial cells stimulated by AT1-AAs. After incubation with AT1-AAs (0.1 μM) for 0, 15, 30, 60, and 120 min and angiotensin II (0.1 μM) for 30 min, p65 levels were determined by Western blot analysis. Panel (a): cytoplasm of p65 levels; panel (b): nucleus of p65 levels. Angiotensin II was used as positive control; versus 0 min (control) group; .

(a)

(b)