Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate
Figure 5
Cytokine production in spleens 48 hours after infection. Mice were vaccinated three times at two-week intervals with 10 μg of either the TetHc-LPS conjugate (∙), LPS alone (■), a mix of unconjugated LPS and TetHc (▲), TetHc only (◆), or PBS (▾) and challenged five weeks after the final vaccination with approximately 40 MLD of B. pseudomallei K96243. Forty-eight hours after challenge, mice were culled and the spleens were removed and assayed for cytokine production. Data is shown for replicate studies and shows the calculated concentration of IL-6 (a), MCP-1 (b), and IFN-γ (c) and was analysed by univariate linear model. Significant differences are shown. Where no markers are present, P was greater than 0.05.
