Construction of a Chimeric Secretory IgA and Its Neutralization Activity against Avian Influenza Virus H5N1
Figure 4
Western blot analysis of recombinant SIgA under nonreducing conditions. Transferred proteins were incubated with different primary antibodies to confirm the various polymeric forms of recombinant SIgA. (a) Mouse anti-human IgA α-chain antibody. (b) Mouse anti-human SC antibody. (c) Mouse anti-human κ-chain antibody. (d) Mouse anti-human J chain antibody. HRP-conjugated goat anti-mouse IgG was used as secondary antibody. Lane 1: produced SIgA; 2: human saliva (positive control). H4L4JSC (complete SIgA molecule), H4JSC/H4L4J (four IgA α-chains, J chain, and secretory component or four IgA α-chains, four κ-chains, and J chain), HJSC/H2L (one IgA α-chain, J chain, and secretory component or two IgA α-chains and one κ-chain), HLJ (one IgA α-chain, one κ-chain, and J chain), LJ (one κ-chain and J chain), and H or SC (monomer of IgA α-chain or secretory component).
