Journal of Immunology Research / 2014 / Article / Tab 3

Research Article

Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies

Table 3

Evaluation of antibody responses in sera collected from chickens vaccinated with a bivalent vaccine (H5/H7) by using the monoplex and multiplex assay formats.

Serum numberHI
H7N1
H7 pp-NT
monoplex
A/ck/Italy/13474/99
H7 pp-NT 
multiplex
(with H5 VN04)
Serum numberHI
H5N9
H5 pp-NT
monoplex
VN04
H5 pp-NT
monoplex
H5 IND05
H5 pp-NT
multiplex
(with H7)

H5+7s1324040H5+7s132578040
H5+7s2165780H5+7s232578028
H5+7s3321010H5+7s364102810
H5+7s4164040H5+7s416284028
H5+7s532453320H5+7s564905453453
H5+7s632226226H5+7s632160160226
H5+7s716113113H5+7s732226320160
H5+7s8165757H5+7s864578057
H5+7s91680113H5+7s932453905320
H5+7s1032226320H5+7s10329059051280

Values are reported as geometric mean titres. Left side: values for H7 A/chicken/Italy/13474/99 tested in monoplex and multiplex are reported. Right side: values for H5 A/Vietnam/1194/04 tested in monoplex and multiplex are reported and the panel of sera was additionally tested against A/Indonesia/5/05.