Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies

Table 3

Evaluation of antibody responses in sera collected from chickens vaccinated with a bivalent vaccine (H5/H7) by using the monoplex and multiplex assay formats.

Serum number	HI; H7N1	H7 pp-NT; monoplex; A/ck/Italy/13474/99	H7 pp-NT; multiplex; (with H5 VN04)	Serum number	HI; H5N9	H5 pp-NT; monoplex; VN04	H5 pp-NT; monoplex; H5 IND05	H5 pp-NT; multiplex; (with H7)
H5+7s1	32	40	40	H5+7s1	32	57	80	40
H5+7s2	16	57	80	H5+7s2	32	57	80	28
H5+7s3	32	10	10	H5+7s3	64	10	28	10
H5+7s4	16	40	40	H5+7s4	16	28	40	28
H5+7s5	32	453	320	H5+7s5	64	905	453	453
H5+7s6	32	226	226	H5+7s6	32	160	160	226
H5+7s7	16	113	113	H5+7s7	32	226	320	160
H5+7s8	16	57	57	H5+7s8	64	57	80	57
H5+7s9	16	80	113	H5+7s9	32	453	905	320
H5+7s10	32	226	320	H5+7s10	32	905	905	1280

Values are reported as geometric mean titres. Left side: values for H7 A/chicken/Italy/13474/99 tested in monoplex and multiplex are reported. Right side: values for H5 A/Vietnam/1194/04 tested in monoplex and multiplex are reported and the panel of sera was additionally tested against A/Indonesia/5/05.