Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling

Figure 4

Kinetics of expression of MAPKs in HEp-2 and A549 cells after inoculation with HPIV-1 virus inactive. ERK1/2, JNK, and p38 phosphorylation showed no time points analyzed during 30 min to 24 hrs. Ctl 2nd lane (−) refers to the proteins of uninfected cells. The Ctl (+) corresponds to the proteins from cells infected with live virus (90 min). Antibodies used are described in Materials and Methods. Western blot assays were performed in triplicate.