Hepatitis E rORF2p Stimulated and Unstimulated Peripheral Expression Profiling in Patients with Self-Limiting Hepatitis E Infection

Table 2

Gene expression quantified using TaqMan Low Density Arrays and normalized with respective controls.

	AVH-E (𝑛=33)	Recovered (𝑛=20)	HEV-specific gene expression
Gene ID and name	Fold change (Mean ± SD)		AVH-E (𝑛=10)	Recovered (𝑛=10)
			72 hrs
Th1/anti-inflammatory cytokines
IL2	1.48±0.46	1.41±0.64	𝟑.𝟖±𝟏.𝟐𝟒	𝟑.𝟕𝟎±𝟏.𝟖𝟏
IFN-𝛾	3.26±1.05	0.62±0.01	𝟎.𝟑𝟐±𝟎.𝟏𝟒	𝟎.𝟓𝟑±𝟎.𝟎𝟕
TGF𝛽1	2.07±0.00	0.51±0.01	𝟑.𝟑𝟎±𝟎.𝟏𝟔	𝟏.𝟑𝟕±𝟎.𝟏𝟒
IL10	11.35±0.30	1.15±0.02	𝟐.𝟔𝟏±𝟎.𝟒𝟑	𝟎.𝟓𝟖±𝟎.𝟎𝟑
IL2R𝛼 (CD25)	8.96±1.04	0.24±0.05	𝟒.𝟓𝟔±𝟎.𝟔𝟎	𝟎.𝟔𝟕±𝟎.𝟎𝟐
Th2/proinflammatory cytokines
IL4	2.11±0.02	0.59±0.01	𝟑.𝟗𝟏±𝟏.𝟗𝟔	𝟎.𝟔𝟕±𝟎.𝟎𝟕
IL6	4.97±0.02	0.28±0.01	𝟏.𝟗𝟗±𝟏.𝟑𝟑	𝟎.𝟓𝟐±𝟎.𝟎𝟒
TNF𝛼	0.87±0.01	0.34±0.02	𝟎.𝟎𝟕±𝟎.𝟎𝟒	𝟎.𝟓𝟏±𝟎.𝟎𝟏
IL17A	8.03±2.64	0.52±0.05	𝟎.𝟐𝟏±𝟎.𝟎𝟐	𝟎.𝟓𝟐±𝟎.𝟎𝟑
Ectonucleotidase
ENTPD1 (CD39)	2.77±0.05	0.18±0.01	𝟎.𝟓𝟗±𝟎.𝟏𝟕	𝟎.𝟔𝟏±𝟎.𝟎𝟑
NT5E (CD73)	0.18±0.00	0.07±0.01	𝟏.𝟔𝟔±𝟎.𝟎𝟔	𝟎.𝟓𝟏±𝟎.𝟏𝟖
Apoptotic/inhibitory
Fas/Apo-1 (CD95)	3.07±0.11	0.53±0.02	𝟎.𝟑𝟓±𝟎.𝟏𝟎	𝟎.𝟓𝟒±𝟎.𝟎𝟒
CTLA-4 (CD152)	6.53±0.22	0.59±0.01	𝟏.𝟓𝟗±𝟎.𝟗𝟗	𝟎.𝟗𝟏±𝟎.𝟎𝟐
PD1 (CD279)	16.80±0.08	0.68±0.00	𝟐.𝟖𝟖±𝟎.𝟒𝟐	𝟎.𝟓𝟑±𝟎.𝟎𝟒
PD1-L (CD274)	2.80±2.39	0.87±0.09	𝟎.𝟑𝟕±𝟎.𝟐𝟏	𝟎.𝟓𝟒±𝟎.𝟎𝟕
GITR (CD357)	4.21±0.07	0.63±0.00	𝟎.𝟖𝟐±𝟎.𝟒𝟎	𝟎.𝟔𝟐±𝟎.𝟐𝟏
Costimulatory
CD40 (CD154)	1.31±0.12	0.61±0.01	𝟎.𝟗𝟐±𝟎.𝟐𝟗	𝟎.𝟔𝟏±𝟎.𝟎𝟓
CD70 (CD27L)	4.90±0.19	0.64±0.00	𝟎.𝟒𝟎±𝟎.𝟎𝟗	𝟎.𝟓𝟖±𝟎.𝟎𝟏
ICOS (CD278)	1.35±0.23	1.10±0.05	𝟏.𝟖𝟏±𝟎.𝟓𝟔	𝟎.𝟓𝟔±𝟎.𝟐𝟑
4-1BB (CD137)	3.56±0.02	1.07±0.03	𝟏.𝟐𝟐±𝟎.𝟕𝟖	𝟎.𝟓𝟔±𝟎.𝟏𝟏
Activation molecules
CD69	1.00±0.02	0.51±0.02	𝟎.𝟏𝟒±𝟎.𝟎𝟑	𝟎.𝟓𝟓±𝟎.𝟎𝟏
TFRC (CD71)	3.55±0.10	0.70±0.01	𝟎.𝟗𝟔±𝟎.𝟑𝟒	𝟎.𝟓𝟎±𝟎.𝟎𝟐
Chemokine and homing receptors
SELL (CD62L)	1.14±0.06	0.19±0.01	𝟎.𝟓𝟖±𝟎.𝟎𝟑	𝟎.𝟓𝟏±𝟎.𝟎𝟒
𝛼E𝛽7 (CD103)	4.10±0.38	0.68±0.10	𝟏𝟑.𝟑𝟕±𝟏.𝟒𝟕	𝟎.𝟓𝟕±𝟎.𝟎𝟏
CCR4 (CD194)	3.07±0.13	0.64±0.01	𝟐.𝟕𝟖±𝟏.𝟔𝟓	𝟎.𝟓𝟐±𝟎.𝟎𝟓
CCR7 (CD197)	2.67±0.14	0.84±0.01	𝟎.𝟕𝟒±𝟎.𝟏𝟓	𝟎.𝟕𝟑±𝟎.𝟎𝟐

In unstimulated PBMCs of AVH-E and recovered individuals, mean fold changes are compared after normalization with healthy control data. HEV rORF2p stimulated in  vitro cultured PBMCs gene expression pattern after normalization with in  vitro cultured unstimulated PBMCs from respective groups (bold). The values shown are the mean fold change compared with unstimulated/stimulated cells of respective groups and are expressed in mean ± standard deviation.