Tumor-Activated TCR𝜸𝜹⁺ T Cells from Gastric Cancer Patients Induce the Antitumor Immune Response of TCR𝜶𝜷⁺ T Cells via Their Antigen-Presenting Cell-Like Effects

Figure 4

Tumor-activated γ δ T cells trigger the cytotoxic effect of autologous CD8⁺ T cells. Autologous CD8⁺ T cells (2 × 10⁵ cells/mL) were cocultured with tumor cells in a 24-well plate for 6 h in the absence or presence of peripheral-derived γ δ T cells or tumor-activated γ δ T cells at a ratio of 1 : 1 : 1. The culture supernatants were collected and the cytotoxic activity of effector cells was measured using an LDH assay. Results are expressed as means ± SD and are representative of three independent experiments. *𝑃<0.01, **𝑃<0.05.