Functional Properties of CD8+ Lymphocytes in Patients with Pleural Plaque and Malignant Mesothelioma
Figure 5
The percentages of perforin-positive cells in CD8+ lymphocytes of HV, PL, and MM groups. (a) Representative histograms of intracellular perforin (solid line) and isotype control (gray) in fresh or stimulated CD8+ lymphocytes. For staining with an isotype control Ab, PBMCs from arbitrary HV samples were used. (b) Representative graph showing the percentage of perforin+ cells in fresh CD8+ lymphocytes. (c) Representative graph showing the percentage of perforin+ cells in PMA/ionomycin-stimulated CD8+ lymphocytes. (d) Representative graph showing the subtracted percentages of perforin+ cells. ((b)–(d)) Horizontal dotted bars indicate the mean percentage. Data represent values from 16 HV individuals, 16 individuals of the PL group, and 14 individuals of the MM group. Significant differences are indicated by asterisks (, ).
