Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases
Figure 1
Comparable phenotypic analysis of peripheral blood NK, NKT, and T cells using saponin- or methanol-based permeabilization. Freshly isolated human PBMCs /mL were fixed using 2% paraformaldehyde, permeabilized in saponin- (left) or methanol- (right) based buffers, and stained with anti-CD3 and anti-CD56 MoAbs. Lymphocytes were gated on forward/side light scatter properties. Discrimination of CD56+CD3− (NK), CD56+CD3+ (NKT), and CD56−CD3+ (T) cells was comparable between the two methods.
