Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases
Figure 6
Simultaneous detection of IFN-γ and p-p38 MAPK expression in CD56+ CD11c+ cell populations. PBMCs were stained with MoAbs against CD56, CD11c, p-p38, and IFN-γ. CD56+ cells were subgated and the percentage of CD11c+ within CD56+ cells is shown. (a) Gated CD56+ cells were plotted for CD11c versus IFN-γ in order to assess IFN-γ expression within CD11c+ cells. (b) Gated CD56+ CD11c+ cells were plotted for IFN-γ versus p-p38 to measure double positivity for p38 and IFN-γ. A significant expression of early IFN-γ is detected within the CD11c+ CD56+ cell subpopulation.
