Research Article

Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo

Figure 3

Evaluation of the toxic effect of warifteine on neutrophils. (a) Isolated neutrophil suspensions were incubated with the indicated doses of warifteine and subsequently stimulated with PMA. Cultures were then incubated with FITC-labeled anti-Gr-1 antibody, which was followed by incubation with a solution of propidium iodide. The samples were analyzed for propidium iodide labeling by flow cytometry. The plots indicate the labeling of cells in the PMN gate (as defined in Figure 2) and are representative of two independent experiments. (b) Isolated neutrophil suspensions were treated for 90 min with PMA. Some cultures also received the indicated doses of warifteine. The cells were then incubated with the XTT dye and XTT reduction by viable cells was determined. Triton X-100-treated cultures were used as positive (dead cells) controls. Statistical analysis showed no significant differences between warifteine-treated and untreated groups. Data are representative of three independent experiments.
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