Efficacy of Primate Humoral Passive Transfer in a Murine Model of Pneumonic Plague Is Mouse Strain-Dependent

Table 1

Genetic primer targets of the PCR test used to ensure integrity of the inoculum. All primer probe design was based on GenBank accession number NC_003143 (Yersinia pestis CO92, complete genome). MGB is minor groove binder/nonfluorescent quencher covalently bound to the 3′ end of the probe. All probes have a 5′ fluorescein (FAM) label.

Gene	Sequence (5′→3′)
HmsF (chromosomal pgm locus)
Forward	CGGAGAAGCCAACGTTCGT
Reverse	TCTTTCACTTTGCGGCAATG
Probe (MGB)	CCGCCTGCACAACG
F1 (pMT1)
Forward	TTGGCGGCTATAAAACAGGAA
Reverse	CACCCGCGGCATCTGTA
Probe (MGB)	CACTAGCACATCTGTTAAC
Pst (pPCP1)
Forward	CGGCAATCGTTCCCTCAA
Reverse	GGTCAGGAAAAAGACGGTGTGA
Probe (MGB)	AACCATGACACGGTAGACT
VAg (pCD1)
Forward	CGGCGGTTAAAGAGAAATGC
Reverse	CATCGCCGAATACACAATGG
Probe (MGB)	TACTGCCATGAACGCC