Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
Figure 4
Specific amplification curves by duplex TaqMan probe real-time PCR. When Pb-H (FAM) and Pb-N (HEX) probes were combined in a duplex real-time PCR system, only the FAM fluorescent signal could be observed when the template was GD HP-PRRSV strain, no FAM signal was detected when the templates were CH-1a PRRSV strain and other viruses (a), and vice versa, only the HEX signal could be collected when the template was CH-1a PRRSV strain (b). When Pb-N (HEX) and Pb-all (FAM) were combined in a duplex real-time PCR system, the Pb-N (HEX signal) probe could only detect PRRSV strain (c), whereas Pb-all (FAM signal) probe could detect both HP-PRRSV and PRRSV strains (d).
