Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

Table 2

Intra- and interassay reproducibility of real-time PCR.

Concentration of standard plasmid (copies/𝜇L)	𝑛	Intra-assay (Cp)			Interassay (Cp)
		Mean	SD	CV (%)	Mean	SD	CV (%)
HP-PRRSV (SYBR)
10^6	3	14.96	0.02	0.13	14.82	0.29	1.96
10^4	3	21.78	0.04	0.18	21.49	0.57	2.65
10^2	3	28.5	0.16	0.56	28.48	0.27	0.95
PRRSV (SYBR)
10^6	3	15.47	0.02	0.13	15.75	0.31	1.97
10^4	3	22.55	0.05	0.22	22.71	0.19	0.84
10^2	3	29.6	0.01	0.03	29.96	0.34	1.13
HP-PRRSV (FAM)
10^6	3	15.9	0.02	0.13	15.98	0.06	0.38
10^4	3	22.51	0.01	0.04	22.63	0.15	0.66
10^2	3	29.71	0.02	0.07	29.59	0.17	0.57
PRRSV (HEX)
10^6	3	16.59	0.15	0.90	16.47	0.04	0.24
10^4	3	23.41	0.09	0.38	23.44	0.37	1.58
10^2	3	29.75	0.04	0.13	29.56	0.28	0.95