Research Article

Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

Table 4

Limit of detection of VWF antigen detected by using latex agglutination test.

Sample/sample numberNeat1 : 21 : 41 : 8
VWF : Ag (%)aLATeVWF : Ag (%)bLATeVWF : Ag (%)cLATeVWF : Ag (%)dLATe

124.2+12.1+6.1+3.0
220.2+10.2+5.1+2.5
330.6+15.3+6.9+3.8
427.6+13.8+6.9+3.4
526.9+13.3+6.7+3.4
629.1+14.6+7.3+3.6
730.3+15.2+7.6+3.8

aVWF : Ag levels in neat sample that was measured by immunoturbidimetric assay using Hemosil von Willebrand Factor Antigen kit from Instrumentation Laboratory, Italy.
b,c,dEstimated VWF : Ag levels in 1 : 2, 1 : 4, and 1 : 8 diluted samples (i.e., by dividing the VWF : Ag levels of neat sample by the dilution factors of 2, 4, and 8, respectively.
ePositive (+) and negative (−) reactions observed when neat and diluted samples were tested by using latex agglutination test.
VWF : Ag: von Willebrand factor antigen; LAT: latex agglutination test.