Ursodeoxycholic Acid Influences the Expression of but Not FoxO1 in Patients with Non-Cirrhotic Primary Biliary Cirrhosis

Figure 3

Functional studies checking the relationship between FoxO1 and p27^kip1 in liver tissue. (a) Pharmacologically induced inactivation of FoxO1 results in suppression of FoxO1 and p27^kip1 gene expression in the experimental Mx-Cre⁺ FoxO1/3/4 mice in comparison to control Mx-Cre⁻ FoxO1/3/4 mice. (b) FoxO1 protein expression is strongly reduced. Somatic deletion of FoxO1 gene did not affect the p27^kip1 protein level. Results are representative of 5-6 independent experiments. Changes in protein levels were determined by densitometry analyses after normalization to β-actin as a control for loading. Bars indicate the mean ± SEM (^∗𝑃<0.05; ^∗∗𝑃<0.01 versus control).

(a)

(b)