Research Article

Immune Imbalance in Nasal Polyps of Caucasian Chronic Rhinosinusitis Patients Is Associated with a Downregulation of E-Selectin

Figure 1

Scatter plots of adhesion molecule expression in nasal polyps compared to associated inferior turbinates using microarray and qPCR. Each single dot shows the relative expression of the target molecule in nasal polyps compared to associated inferior turbinates of one patient and median is indicated as horizontal bar. (a) Microarray analysis: nasal polyp tissues showed higher gene expression levels of the adhesion molecules P-selectin (1.62-fold, ), PSGL1 (1.74-fold, ), and VCAM1 (2.16-fold, ) compared to associated inferior turbinates. ICAM1 was unregulated (1.03-fold, ) and E-selectin was strongly downregulated in nasal polyps (0.32-fold, ). (b) qPCR analysis: lower median expression of E-selectin (0.22-fold, ) and higher median expression of P-selectin (2.37-fold, ) were observed in nasal polyps. The median expression of PSGL1, ICAM1, and VCAM1 showed no significant difference between nasal polyps and inferior turbinates: PSGL1 1.24-fold ( ), ICAM1 1.24-fold ( ), and VCAM1 0.85-fold ( ). The mRNA levels of E-selectin in nasal polyps decreased significantly ( ) compared to the inferior turbinates and P-selectin increased significantly ( ). PSGL1, ICAM1, and VCAM1 were expressed at similar levels.
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(a)
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(b)